ABSTRACT
Fasciola hepatica somatic antigen, its partially purified fractions and excretion-secretion products were investigated as to serological, electrophoretic and biological properties. In a Sephadex G-100 column (SG-100), Fasciola hepatica total antigen (FhTA) gave 5 fractions, and SDS-PAGE analysis showed they were glycoproteins ranging from 14 to 94 kDa molecular weight (MW). When these fractions were analyzed by enzyme-linked immunotransfer blot (EITB) and immunodiffusion in gel (ID) with serum from immunized rats with FhTA, the presence of different antigenic components was revealed. In the SDS-PAGE of excretor-secretor antigen (ESA), it was possible to observe peptides from 12 to 22 kDa, which were also present in FhTA. When the FhTA, its fractions and the ESA were analyzed by EITB with the immune rat serum (IRS), it was observed that only some fractions of the SG-100 shared antigens with the FhTA and ESA. Moreover, DTH and ITH responses were studied in FhTA immunized rats challenged with these different antigen components, revealing that the protein/carbohydrate ratio is important for inducing DTH response. The ESA was the most active component in the DTH and ITH response
Subject(s)
Animals , Antigens, Helminth/analysis , Fasciola hepatica/immunology , Antigens, Helminth/immunology , Antigens, Helminth/isolation & purification , Chromatography, Gel , Electrophoresis, Polyacrylamide Gel , Enzyme-Linked Immunosorbent Assay , Hypersensitivity, Delayed/immunology , Hypersensitivity, Immediate/immunology , Immunoblotting , ImmunodiffusionABSTRACT
The objective of the present work was to carry out a survey of soil samples taken from different areas of a hospital of infectious disease located in the city of Córdoba, where three AIDS patients were hospitalized during different periods in the same ward. The three of them returned with meningeal cryptococcosis between three or five months after having been discharged. Cryptococcus neoformans was isolated in 8/10 samples collected outside the hospital, near the pigeon house. The samples collected from the AIDS patients ward and its surroundings were negative. These findings suggest that the patients may have been infected by the fungus during their first stay in hospital
Subject(s)
Humans , AIDS-Related Opportunistic Infections/etiology , Cross Infection/etiology , HIV-1 , Meningitis, Fungal/etiology , Adult , AIDS-Related Opportunistic Infections/microbiology , AIDS-Related Opportunistic Infections/transmission , Argentina , Cross Infection/microbiology , Cross Infection/transmission , Cryptococcosis/etiology , Cryptococcosis/microbiology , Cryptococcosis/transmission , Cryptococcus neoformans/isolation & purification , Environmental MicrobiologyABSTRACT
Se presenta un estudio epidemiológico para histoplasmosis, coccidioidomicosis y criptococosis realizado en cinco zonas de la provincia de Córdoba. Los datos obtenidos demonstraron una positividad global de 41.1% para histoplasmina, 26.7% para coccidioidina y 14.1% para criptococcina. En algunas regiones, cuenca del Río III y Traslasierra los índices de infección para histoplasmosis fueron mucho más altos, 53.3% y 73.1% respectivamente. Também las intradermorreacciones para criptococosis en Traslasierra fueron muy elevados: 31,9%
Subject(s)
Adolescent , Adult , Middle Aged , Humans , Male , Female , Coccidioidomycosis/epidemiology , Cryptococcosis/epidemiology , Histoplasmosis/epidemiology , Lung Diseases, Fungal/epidemiology , Antigens, Fungal , Argentina , Coccidioidin , Histoplasmin , Intradermal TestsABSTRACT
En el período comprendido entre 1977 y 1982, desde que se constituyó el equipo actual de Micología dela Cátedra, han sido diagnosticados y controlados 4 casos de Histoplasmosis, 2 de Coccidioidomicosis, 1 Actinomicosis, 1 Nocardiosis, 1 Criptococcosis y 28 Aspergilosis. Las características comunes a las micosis causada por Histoplasma y Coccidioides han sido la presentación clínica solapada, crónica, con imágenes radiológicas cavitarias que despertaron la sospecha de tuberculosis en su comienzo. Los Aspergilomas intracavitarios se manifestaron en todos los casos por hemoptisis de duración variable en pacientes con secuelas de T.B.C. graves. Las imágenes radiológicas de esta enfermedad difirieron de la descripción clásica en cuanto no se halló más que en un caso el típico ovillo intracavitario y en cambio lo más frecuente fue el pulmón destruido o las cavernas de opacificación gradual. En la Criptococcosis y en uno de los casos causados por Actinomycetales la clínica y la radiología fueron compatibles con cáncer de pulmón y el diagnóstico fue hecho luego de cirugía mediante estudio micológico de las piezas de biopsia. El objetivo de la presentación de estos datos es llamar la atención de los médicos generales y neumonólogps acerca de estas enfermedades que no suelen ser tenidas en cuenta en los diagnósticos diferenciales